Alpha-amylase assay with dyed-starch in polyethylene glycol and dextran solutions

✍ Scribed by Kyung Moon Park; Nam Sun Wang

• Book ID: 104644851
• Publisher: Springer-Verlag
• Year: 1991
• Tongue: English
• Weight: 227 KB
• Volume: 5
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00152782

✦ Synopsis

The a-amylase assay method with RBB-starch was shown to work well in the presence of polyethylene glycol and dextran, polymers commonly used in aqueous two-phase systems. Neither the polymer type, polymer concentration, nor molecular weight affected the a-amylase activity measurement. On the other hand, the same polymers significantly interfered with the Nelson copper method, the DNS method, and the iodine-starch method.