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Allosteric inhibition of the DNA-dependent ATPase activity of Escherichia coli DNA gyrase by a representative of a novel class of inhibitors

โœ Scribed by Adam B. Shapiro; Beth Andrews


Book ID
119194817
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
372 KB
Volume
84
Category
Article
ISSN
0006-2952

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A system for detecting a spontaneous deletion in Escherichia coli was developed and the role of DNA gyrase in deletion formation was studied. A derivative of 2plac5, 2AM36, was isolated in which whole pBR322 DNA was inserted in the lacZ gene and 227 bp of the lac gene duplicated at both sides of the