Allogeneic blood transfusion reduces murine pulmonary natural killer (NK) activity and enhances lung metastasis of a syngeneic tumour
✍ Scribed by P. Jane Clarke; Robert C. Burton; Kathryn J. Wood
- Book ID
- 102864438
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- French
- Weight
- 773 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
A model was established whereby C57BL/6 (66) blood injected i.v. into C3H mice 7 days prior to i.v. injection of syngeneic UV-2237 tumour cells significantly increased the number of pulmonary metastases counted 21 days later as compared with levels observed in mice treated with saline, C3H or NZW blood or SRBC. This regimen of 66 allogeneic blood transfusion of C3H mice also significantly depressed splenic and pulmonary NK activity as assayed by lysis of "Cr YAC-I in vitro and by clearance of "'In YAC-I in vivo respectively. Anti-asialo GM I treatment, which depletes NK activity in vivo, and Poly I:C treatment, which enhances NK activity in vivo, were associated with significantly increased and decreased pulmonary metastasis of UV-2237, respectively, in C3H mice. Depletion of CD4+ and CD8+ T cells had no effect. Cyclophosphamide pretreatment which, among other effects, depletes NK cells, significantly increased pulmonary metastasis of UV-2237 in C3H mice. This was corrected by adoptive transfer of normal C3H spleen cells but not spleen cells from anti-asialo GM I -treated C3H mice or 66-blood-transfused C3H mice. Furthermore, a I : I mixture of normal C3H spleen cells with spleen cells from 66-blood-transfused C3H mice also failed to reconstitute the cyclophosphamide-pre-treated C3H mice. We conclude that allogeneic blood transfusion augments pulmonary metastasis of the UV-2237 sarcoma in C3H mice and that the mechanism involves suppression of N K activity.