Allo-class i-reactive cd4-cd8- t cell hybridomas recognize the conformational change of class i molecule resulting from the exchange of the α3 domain

✍ Scribed by Junji Yamamoto; Manabu Tanabe; Mutsuhiko Minami; Kyoichi Kano; Masafumi Takiguchi

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 474 KB
• Volume: 19
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830191016

✦ Synopsis

Allo-class I-reactive CD4-CDtt T cell hybridomas recognize the conformational change of class I molecule resulting from the exchange of the a3 domain*

H-2Kb-reactive, interleukin (IL) 2-producingTcel1 hybridomas possessing neither CD8 nor CD4 molecules were employed for the study of allorecognition on interspecies hybrid antigens by T cells in the absence of an influence of these accessory molecules. Both HTB176.10 and HTB177.2 Tcell hybridomas reacted with KbKbB7 hybrid antigens as well as Kb antigens and they produced more IL 2 in response to Kb antigens than KbKbB7 hybrid antigens. IL2 production of these Tcell hybridomas was dependent on the surface expression level of Kb molecules on stimulators. Therefore, L cells expressing almost equivalent levels of Kb or hybrid antigens were selected for further functional studies by these T cell hybridomas. They apparently produced less IL2 in response not only to interspecies hybrid antigens but also to interspecies hybrid antigens in response to Kb antigens. These results indicated that T cell hybridomas recognized the conformational change of class I molecule resulting from the exchange of the a3 domain via their T cell receptors.