Allergy to drugs: antioxidant enzymic activities, lipid peroxidation and protein oxidative damage in human blood
✍ Scribed by José M. Matés; Cristina Pérez-Gómez; Lucia Olalla; José M. Segura; Miguel Blanca
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 126 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0263-6484
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✦ Synopsis
Reactive oxygen species lead to lipid peroxidation and speci®c oxidation of some speci®c enzymes, proteins and other macromolecules, thus aecting many intra-and intercellular systems. Recently, antioxidant functions have been linked to anti-in¯ammatory properties. Cell defences against toxic oxygen include antioxidant enzymes. We studied the enzymic antioxidant capacity in human blood of both erythrocytes and mononuclear cells from patients suering from an allergic reaction to dierent drugs. We determined superoxide dismutases (SODs), glutathione peroxidase (GSHPx) and catalase (CAT) activities in each cell type. We also determined the extent of thiobarbituric acid reactive substances (TBARS) and the oxidative damage to proteins, in order to study the correlation between the cellular enzymic activities, the oxidative status and the allergic reaction. In mononuclear cells from allergic patients, SODs and CAT activities were enhanced compared with controls. Conversely, a decrease in GSHPx activity was found. In erythrocytes, higher values for CAT, GSHPx and SODs activities were found in allergic patients. TBARS were also enhanced in both types of cells, and the carbonyl content of serum was equally increased. The respective enzymic imbalances in mononuclear cells and erythrocytes, namely, GSHPx/SOD and CAT/SOD, and their consequences are discussed. To our knowledge, this is the ®rst global study of antioxidant enzyme determinations, including TBARS level and carbonyl content, in patients suering from allergies to drugs.