Allergen extracts directly mobilize and activate human eosinophils

✍ Scribed by Lena Svensson; Anna Rudin; Christine Wennerås

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 173 KB
• Volume: 34
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.200324798

✦ Synopsis

Abstract

Allergic diseases are characterized by the presence of eosinophils, which are recruited to the affected tissues by chemoattractants produced by T cells, mast cells and epithelium. Our objective was to evaluate if allergens can directly activate human eosinophils. The capacity of purified allergen extracts to elicit eosinophil chemotaxis, respiratory burst, degranulation and up‐regulationof the adhesion molecule complement receptor 3 (CR3) was determined in eosinophils isolated from healthy blood donors. Eosinophils stimulated with an extract from house dust mite (HDM) released thegranule protein major basic protein (MBP) and up‐regulated the surface expression of CR3. Cat allergen extracts also induced the up‐regulation of CR3, but not the release of MBP; instead cat, as well as birch and grass allergens, elicited the release of eosinophil peroxidase (EPO). In addition, grass pollen extract caused the secretion of MBP. None of the allergens stimulated eosinophilic cationic protein release, nor production of free oxygen radicals. Both HDM and birch extracts were chemotactic for eosinophils. These findings establish that common aeroallergens can directly activate eosinophils in vitro. We propose that eosinophil activation in vivo is not exclusively mediated by cytokines and chemokines of the allergic inflammatory reaction, but could partly be theresult of direct interaction between allergens and eosinophils.