Allelic imbalance within the E-cadherin gene is an infrequent event in prostate carcinogenesis
✍ Scribed by Susan J. Murant; Nicky Rolley; Stewart M.A. Phillips; Michael Stower; Norman J. Maitland
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 139 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1045-2257
No coin nor oath required. For personal study only.
✦ Synopsis
By exploiting two single nucleotide polymorphisms (SNPs) located within the E-cadherin gene, at 16q22, we have determined the frequency of allelic imbalance at this proposed tumor suppressor locus in a series of human prostatic carcinoma DNA samples. Whereas results with seven highly polymorphic microsatellite markers flanking the E-cadherin locus confirmed the existence of three separate loci on chromosome 16, at which allelic imbalance increased with increasing loss of tumor cell differentiation, no allelic imbalance within the E-cadherin gene was detected either by single-strand conformational polymorphism analysis or by direct sequencing. We conclude that the loss of E-cadherin function observed in prostate cancer is not a result of allelic deletion.