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Allelic drop-out and preferential amplification in single cells and human blastomeres: implications for preimplantation diagnosis of sex and cystic fibrosis

โœ Scribed by Findlay, lan; Ray, Pierre; Quirke, Phil; Rutherford, Anthony; Lilford, Richard


Book ID
111872953
Publisher
Oxford University Press
Year
1995
Tongue
English
Weight
871 KB
Volume
1
Category
Article
ISSN
1360-9947

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The combination of in vitro fertilization (IVF) with PCR technologies enables diagnosis of single gene defects for preimplantation genetic diagnosis. This has been accomplished by two-step nested PCR, or PEP-PCR followed by nested PCR processes. To improve the detection of single cell genetic defect