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Alkylation of RNA by vinyl chloride metabolites in vitro and in vivo: Formation of 1-N6-etheno-adenosine

✍ Scribed by R.J. Laib; H.M. Bolt


Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
518 KB
Volume
8
Category
Article
ISSN
0300-483X

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πŸ“œ SIMILAR VOLUMES


Alkylation of RNA by vinyl bromide metab
✍ H. OttenwΓ€lder; R. J. Laib; H. M. Bolt πŸ“‚ Article πŸ“… 1979 πŸ› Springer-Verlag 🌐 English βš– 374 KB

[1,2-14C]Vinyl bromide was incubated with rat liver microsomes, NADPH, and polyadenylic acid, polycytidylic acid, or RNA, respectively. Part of the adenosine moieties in RNA or in polyadenylic acid were alkylated and labelled 1,N6-ethenoadenosine structures were formed. Part of the cytidine moieties

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Rats were exposed to [1,2-14C] vinyl chloride. Liver RNA was isolated, hydrolyzed, and the nucleosides separated on Aminex-A-6. Besides the physiological bases and 1,N6-ethenoadenosine, radioactivity was also incorporated into 3,N4-ethenocytidine. Radioactive 3,N4-ethenocytidine moieties were also f