Alkalosis monitored by 31P NMR in a human glioma cell line exposed to the anti-tumor drug 1, 3-bis (2-chloroethyl)-1-nitrosourea
✍ Scribed by Fernando Commodari; Bryan C. Sanctuary; William Feindel; Eric A. Shoubridge
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 570 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
A transient alkalosis of similar magnitude to that observed in vivo has been observed using ^31^P NMR and 2‐deoxy‐D‐glucose‐6‐phosphate as a pH marker in a human glioma cell line, SKI‐1, with demonstrated sensitivity to 1, 3‐bis (2‐chloroethyl)‐1‐nitrosourea. At an effective dose of 5 ± 1 × 10 μg/ml, an increase of 0.13 ± 0.05 pH units was observed within 4 ± 1 × 10 min of introducing the drug into the perfusion chamber. Although the in vitro response is of a time course much faster than that in vivo, these results suggest that this immediate pH change could be an indicator of the cytotoxic action of the drug. © 1991 Academic Press, Inc.