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Alkaloid Production in Catharanthus Roseus Cell Cultures. XI. Biotransformation of 3′, 4′-Anhydrovinblastine to Other Bisindole Alkaloids

✍ Scribed by James P. Kutney; Barbara Aweryn; Lewis S. L. Choi; Pawel Kolodziejczyk; Wolfgang G. W. Kurz; Kenneth B. Chatson; Friedrich Constabel


Publisher
John Wiley and Sons
Year
1982
Tongue
German
Weight
489 KB
Volume
65
Category
Article
ISSN
0018-019X

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✦ Synopsis


Abstract

Biotransformation studies of 3′,4′‐anhydrovinblastine (1) in Calharanthus roseus cell suspension cultures are reported. A detailed study of one cell line, coded at „916”︁, has revealed that 1 is transformed to the natural bisindole alkaloids leurosine (2) and Catharine (3) in approximately 25–30 and 9–16% yield, respectively. In 24–48 h, about 40% of 1 is utilized by the cells. Conditions for biotransformation of 1 have not been optimized so the above yields quoted are not final.