Alkaline phosphatase-modified carbon fibre disc microelectrode for the determination of 4-aminophenyl phosphate
✍ Scribed by F. Pariente; L. Hernández; H.D. Abruña; E. Lorenzo
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 560 KB
- Volume
- 273
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
Madnd &am) (Recewed 1st June 1992, rewed manuscript recewed 23rd September 1992) Ah&act The construction and response of an alkalme phosphatase-modtied carbon fibre disc nucroelectrode and its use as an amperometnc sensor for the determmatlon of 4-ammophenyl phosphate are described The enzyme was mnnoblllzed on the electrode surface usmg glutaraldehyde and bovme serum albumin as cross-hnkmg agents The product of the enzymatic reaction, 4-ammophenol, was determined by oxidation at +0 3 V vs a sodium chloride saturated calomel electrode, yleldmg a steady-state current directly related to the bulk concentration of the substrate The response time of this rmcrobmsensor was typically less than 3 s and 4-ammophenyl phosphate could be deternuned m the range 5 0 X 10e7-5 0 X 10m3 M Keywords Amperometry, Blosensors, Enzymatic methods, Alkalme phosphatase-moddied electrode, Ammophenyl phosphate, Carbon fibre disc nucroelectrode, Enzyme electrodes