Aliphatic alcohols improve the adsorptive performance of cellulose nitrate membranes—Application in chromatography and enzyme assays
✍ Scribed by Zenon Schneider
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 570 KB
- Volume
- 108
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Cellulose nitrate membranes of some sources adsorb reversibly adenine, cytosine, and benzimidazole. The performance of the membranes depends on the manufacturer and is affected by storage conditions or artificial aging. The treatment of the membranes with aqueous solutions of isobutyl alcohol and other aliphatic alcohols at 65°C improve significantly their affinity toward adenine. The alcohol-treated membranes retain their high performance for 2 years if kept at room temperature. Kinetic studies at different pH revealed that the optimum of adsorption and desorption rate falls between pKol and pK,, values of adenine. The rate constants of adsorption at pH 5.75, 3.35, and 2.50 have been found to be 0.590 min-I, 0.156 min-I, and 0.095 min-*, respectively. Appreciable concentrations of salts do not affect significantly the adsorptive affinity of the alcohol-treated cellulose nitrate membranes. The membranes may be applied to rapid estimation of tiny amounts of a number of enzymes by means of chromatography on strips 8 x 40 mm in size.