Alginate-based solid media for plant tissue culture

✍ Scribed by Kurt Ingar Draget; Kjetill Østgaard; Olav Smidsrød

• Book ID: 104683236
• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 426 KB
• Volume: 31
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00252532

✦ Synopsis

A new method for solid medium plant tissue culture based on in situ gelation of alginate is proposed as an alternative to agar-based media. In situ gelation by the use of dispersed CaCO3 and the slowly hydrolysing acid glucono-6-1actone (GDL) was the basis for the use of alginate as a gelling agent. Inexpensive alginate-based media can be made in a wide range of pH values. Biological tests of these gels, concerning sterile seed growth and microcalli plating of Brassica napus (cv. Topas) and biomass production of Panax 9insen9 callus, showed results equal to those achieved with agar-based gels.