Alcohol dehydrogenase-coupled spectrophotometric assay of plasmalogenase
โ Scribed by Neil M. Freeman; Eric M. Carey
- Book ID
- 107711873
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 547 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
\(\alpha\)-Ketoaldehydes have been extensively employed as reagents for the chemical modification of arginine residues in proteins, and to probe for putative anion recognition sites. A major disadvantage in their use is instability, as \(\alpha\)-ketoaldehydes are prone to hydration, oxidation, and
A simple spectrophotometric method for estimation of diamine oxidase (DAO) activity was developed, based on a coupled reaction with NADH-dependent alcohol-dehydrogenase. Cystamine, upon DA0 action, is transformed into an aminoaldehyde which reacts quickly and quantitatively with NADH in the presenc