ALBUMIN CAN REVERSE THE RELEASE OF POTASSIUM FROM HUMAN ERYTHROCYTES TREATED WITH THE NON-IONIC DETERGENT, BRIJ 58

✍ Scribed by PETER BOGNER; DENYS N. WHEATLEY; CSABA BORBÉLY; ATTILA MISETA

• Book ID: 102565713
• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 322 KB
• Volume: 20
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1996.0096

✦ Synopsis

Exchange of erythrocyte intracellular (i/c) K + for extracellular (e/c) Na + in human erythrocytes treated with sub-CMC concentrations of the non-ionic detergent Brij 58 can be stopped by reincubation in serum or albumin containing solutions. The progressive equilibration of the K + contents of detergent-treated human erythrocytes with the incubation medium was reversed by an albumin-mediated withdrawal of detergent molecules from the cell. Re-establishment of near normal [K + ] in terms of K + /kg water proceeds in two ways: (i) a metabolism-dependent net accumulation of K + ions; and (ii) a metabolism-independent shrinkage of erythrocytes, this being the more significant factor.