Albumin absorption and protein secretion by the gallbladder in man and in the pig

✍ Scribed by Julius L. Toth; P. Robert C. Harvey; G. Aravinda Upadyha; Steven M. Strasberg

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 961 KB
• Volume: 12
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.1840120418

✦ Synopsis

To study albumin absorption by the gallbladder in man, an in uitro model was first established in the pig and compared with in uiuo function in the same species. Water and electrolyte transport and '%Ialbumin absorption and protein secretion in uiuo and in uitro were compared. Then similar in uitro studies were performed on human gallbladders obtained at surgery. The in uiuo study in the pig wa8 performed without disturbing the gallbladder except to tie a c m d a in the cystic duct end. The in u i h model was identical in the pig and human gallbladders. Gall- bladders were excised using a technique causing minimal hjury and anoxia. They were oxygenated on both mucosal and serosal surfaces in a temperaturecontrolled environment. Luminal and external bath test solutions consisted of modified Ringers bicarbonate with added glucose; luminal solutions also contained "'I-albumin from different species, depending on the study. Active absorption of sodium and water occurred in both types of studies in the pig but in uiuo absorption rates were considerably greater than in uitro rates. Albumin absorption in uiuo was substantial; although present in uitro, the absorption of albumin was diminished relatively more than electrolyte transport rates. Protein secretion rates into the gallbladder were similar in uitro and in viuo. The results of studies in the human gallbladders in uitro were similar to the pig, except albumin absorption was greater. Some human gallbladders were obtained from control patients and some from patients with cholesterol gallstones. There were no significant &f'€erencee between the two groups for any of the variables studied; however, the numbers were small and some control gallbladders were not normal gallbladders.