Alanyl-tRNA synthetase of Escherichia coli: Genetic analysis of the structural gene and of suppressor mutations
✍ Scribed by Ruffler, Dieter ;Buckel, Peter ;Piepersberg, Wolfgang ;Böck, August
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 584 KB
- Volume
- 134
- Category
- Article
- ISSN
- 0026-8925
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Among temperature resistant revertants of a temperature sensitive E. Coli alanyl-tRNA synthetase mutant a strain was found which contains an alanyl-tRNA synthetase with an additional mutation in the structural gene of the enzyme. This mutant enzyme has a 9 or 38 fold decreased Km value for alanine c
Spontaneous revertants of a temperature-sensitive Escherichia coli strain harboring a thermolabile leucyl-tRNA synthetase and seryl-tRNA synthetase were selected for growth at 40 degrees C. Among these, strains were found with increased levels of both thermolabile synthetases. Two distinct genetic l