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Alanine Scanning Mutagenesis of Insulin

✍ Scribed by Kristensen, C.; Kjeldsen, T.; Wiberg, F. C.; Schaffer, L.; Hach, M.; Havelund, S.; Bass, J.; Steiner, D. F.; Andersen, A. S.

Book ID
124096869
Publisher
The American Society for Biochemistry and Molecular Biology
Year
1997
Tongue
English
Weight
758 KB
Volume
272
Category
Article
ISSN
0021-9258

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## Abstract Alanine scanning mutagenesis of protein–protein interfacial residues can be applied to a wide variety of protein complexes to understand the structural and energetic characteristics of the hot‐spots. Binding free energies have been estimated with reasonable accuracy with empirical metho