Al2O3 doped apatite-wollastonite containing glass ceramic provokes osteogenic differentiation of marrow stromal stem cells

✍ Scribed by Ohgushi, H. ;Yoshikawa, T. ;Nakajima, H. ;Tamai, S. ;Dohi, Y. ;Okunaga, K.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 787 KB
• Volume: 44
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(19990315)44:4<381::aid-jbm3>3.0.co;2-e

✦ Synopsis

Fresh marrow cells were obtained from femora of Fischer rats and cultured in a medium containing 15% fetal calf serum (FCS) until confluence. After trypsinization, cells were subcultured at a cell density of 100 × 10 3 /35-mm well in the presence of FCS, ␤-glycerophosphate, and ascorbic acid phosphate on four different culture substrata. The period of subculture was 2 weeks; the substrata used were the culture dish, apatite-wollastonite containing glass ceramic (AW), hydroxyapatite coated AW (HA/AW), and Al 2 O 3 doped AW (Al/AW). The HA coating was attained by the incubation of AW in simulated physiological solution. The glass matrix of AW and HA/AW contained MgO, CaO, P 2 O 5 , and SiO 2 ; Al/AW contained Al 2 O 3 in addition to these components. The subculture on Al/AW substratum showed many alkaline phosphatase (ALP) positive nodules and the highest ALP activity. On a Northern blot analysis the housekeeping gene of ␤-actin mRNA was evenly detected from the cells cultured on all substrata; however, bone-specific osteocalcin mRNA was only detected from the cells on Al/ AW. These results indicate that Al/AW provokes the osteoblastic differentiation of marrow stromal stem cells.