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Air–Liquid Interface Culture of Nasal Epithelial Cells on Denuded Amniotic Membranes

✍ Scribed by Nurit Even-Tzur; Ariel Jaffa; Zoya Gordon; Ruth Gottlieb; Yoel Kloog; Shmuel Einav; Michael Wolf; David Elad


Book ID
107620726
Publisher
Springer
Year
2010
Tongue
English
Weight
957 KB
Volume
3
Category
Article
ISSN
1865-5025

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Serially passaged normal human bronchial epithelial (NHBE) cell monolayers were established on Transwell inserts via an air-liquid interface (ALI) culture method. NHBE cells were seeded on polyester Transwell inserts, followed by an ALI culture from day 3, which resulted in peak TEER value of 766+/-