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Agrobacterium tumefaciens-mediated transformation ofAspergillus aculeatusfor insertional mutagenesis

✍ Scribed by Emi Kunitake; Shuji Tani; Jun-ichi Sumitani; Takashi Kawaguchi

Book ID
115028173
Publisher
Springer-Verlag
Year
2011
Tongue
English
Weight
260 KB
Volume
1
Category
Article
ISSN
2191-0855

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Genetically transformed barley was produced by eco‐cultivating immature embryo explants with __Agrobacterium tumefaciens__ carrying a binary vector coding for chimaeric bacterial genes, __bar__ and __gus__, and selecting for bialaphos‐resistant cultures from which plants were regenerated. Integratio