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Agrobacterium-mediated Transformation of Sunflower (Helianthus annuusL.): A Simple Protocol

✍ Scribed by K.SANKARA RAO; V.K. ROHINI


Book ID
115570864
Publisher
Oxford University Press
Year
1999
Tongue
English
Weight
364 KB
Volume
83
Category
Article
ISSN
0305-7364

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Transformation of sunflower (Helianthus
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A procedure was developed for transformation of Helianthus annuus (sunflower) using Agrobacterium tumefaciens. Cotyledons were removed from young seedlings, and the remaining tissue was uniformly wounded by shaking with glass beads. The wounded tissue was then co-cultivated with a hypervirulent stra