Agrin and acetylcholine receptors are removed from abandoned synaptic sites at reinnervated frog neuromuscular junctions
✍ Scribed by Stanco, Anne M. ;Werle, Michael J.
- Book ID
- 102655084
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 787 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
Changes in the distribution of agrin tion to a punctate, pale pattern, and finally becoming and acetylcholine receptors (AChRs) were examined entirely absent. Agrin and AChRs were removed from during reinnervation and following permanent denerabandoned synaptic sites in reinnervated frog neurovation as a means of understanding mechanisms conmuscular junctions, while in contralateral muscles trolling the distribution of these molecules. Following which were permanently denervated, anti-agrin and nerve damage in the peripheral nervous system, re-AChR staining remained at abandoned synaptic sites. generating nerve terminals preferentially return to Decreasing synaptic activity during reinnervation deprevious synaptic sites leading to the restoration of layed the removal of agrin and AChRs from abansynaptic activity. However, not all portions of original doned synaptic sites. Altogether, these results support synaptic sites are reoccupied: Some of the synaptic the hypothesis that synaptic activity controls a cellular sites are abandoned by both the nerve terminal and mechanism that directs the removal of agrin from the Schwann cell. Abandoned synaptic sites contain synaptic basal lamina and the loss of agrin leads to agrin, AChRs, and acetylcholinesterase (AChE) withthe dispersal of AChRs. ᭧ 1997 John Wiley & Sons, Inc. J out an overlying nerve terminal or Schwann cell pro-Neurobiol 33: 999-1018, 1997 viding a unique location to observe changes in the Keywords: agrin; acetylcholine receptors; neuromusdistribution of these synapse-specific molecules. The cular junction; reinnervation; abandoned synaptic distribution of anti-agrin and AChR staining at abansites doned synaptic sites was altered during the process of