✦ LIBER ✦

Aggregation of chemically dissociated sponge cells in the absence of protein synthesis

✍ Scribed by Humphreys, Tom

Publisher: John Wiley and Sons
Year: 1965
Tongue: English
Weight: 432 KB
Volume: 160
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401600211

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✦ Synopsis

Puromycin and ethionine immediately inhibit protein synthesis more than 90% in chemically dissociated sponge cells. Initial aggregation proceeds normally in the presence of both inhibitors, indicating that protein synthesis is not necessary for the aggregation of these cells, After six hours in puromycin, the cells stop aggregating and die. In ethionine, the cells aggregate and remain aggregated for at least 48 hours, although protein synthesis remains repressed. Thus protein synthesis is not necessary for producing the material which binds these cells together. Although the aggregation is not dependent on concurrent protein synthesis, it was conceivable that protein which was stored in the cell was secreted during aggregation. However, sponge cells could aggregate over and over in ethionine, indicating that the cells were not dependent on a stored supply of material.

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