Aggregation of calcium oxalate crystals: Effect of urine and various inhibitors

✍ Scribed by Felix, R. ;Monod, A. ;Broge, L. ;Hansen, N.M. ;Fleisch, H.

• Publisher: Springer
• Year: 1977
• Tongue: English
• Weight: 570 KB
• Volume: 5
• Category: Article
• ISSN: 0300-5623
• DOI: 10.1007/bf00257112

✦ Synopsis

The influence of various factors on aggregation of calcium oxalate crystals in vitro was determined. Aggregation was assessed by filtering the crystal suspension and measuring the flow rate through a filter. 10% urine completely inhibited aggregation. Orthophosphate and magnesium at concentrations occuring in urine had no effect. Citrate had no effect at 10(-4) M ,but did inhibit at 10(-3) M. The latter effect is probably due to calcium binding. Pyrophosphate and disodium dichioromethylene diphosphonate (C12MDP) inhibited strongly at 10(-4) M, disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP) at 10(-5) M, whereas pentanemonophosphonate had no effect. Uromucoid also did not show any inhibitory activity. Studies by means of heat inactivation, ultrafiltration and fractionation on DEAE-cellulose and gel-filtration indicated that the inhibitory activity was heterogenous and that the major part was larger than 10 000 daltons.