Age-related changes in hematopoietic stem cell populations of a long-lived hybrid mouse
✍ Scribed by Michael G. Chen
- Publisher
- John Wiley and Sons
- Year
- 1971
- Tongue
- English
- Weight
- 652 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Age-related changes in the number and concentration of pluripotential and unipotential hematopoietic stem cells in the femoral bone marrow and spleen of BC3FI mice were investigated. Pluripotential stem cells were assayed by the spleen colony technique, and unipotential stem cells were determined by an agar cloning method and by erythropoietin responsiveness in polycythemic mice. Changes with senescence were observed in the concentration of both uni-and pluripotential stem cells in the bone marrow; the size of the stem cell compartment in the marrow did not change significantly with age. Also, a reduction in the seeding of transplanted spleen colony-forming units into the spleens of aged recipients was demonstrated. The implications of these findings for the kinetics of hematopoietic stem cell proliferation in aged animals are discussed.