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Age estimation using a modified HPLC determination of ratio of aspartic acid in dentin

✍ Scribed by Shi-Jiang Fu; Chui-Chang Fan; Hong-Wei Song; Feng-Qun Wei

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
232 KB
Volume
73
Category
Article
ISSN
0379-0738

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✦ Synopsis

Diastereomeric dipeptides were derived from the amino acid enantiomers in dentin by O-phthalaldehyde-N-acetyl-L-cysteine. The products were separated using high performance liquid chromatography (HPLC) and detected by fluorescence detector. A short analysis time (total analysis time was 15 min, including retention time, sample derivatization time and column regeneration time) was used. The sensitivity of detection was 1 pmol and high resolution (Rs = 1.5) was reached. We determined the D/L ratio of aspartic acid in dentin of 28 first premolars. The correlation value between the D/L ratio of aspartic acid and actual age was 0.9887; errors of +/- 1 year accounted for 46.4%, and no error exceeded 5 years.

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## Abstract The D/L ratio of aspartic acid enantiomers in proteins of low turnover is generally accepted as a reliable procedure for age determination. In our study, twelve samples of eyetooth dentin were analyzed for age determination. The pure insoluble collagen isolated from eyetooth dentin was