Age distribution of antibodies to human papillomavirus in children, women with cervical intraepithelial neoplasia and blood donors from South Africa
✍ Scribed by Marais, Dianne; Rose, Robert C.; Williamson, Anna-Lise
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 54 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
a significant role in the development of cervical intra-Sera from 95 women with cervical intraepithelial epithelial neoplasia (CIN) and cervical cancer [Schiffneoplasia (CIN), 95 age-matched female blood man, 1992]. South Africa has an extremely high incidonors, and 155 children aged between 1 and 12 dence of cancer of the cervix and at least 4.7% of black years were tested by enzyme-linked immunosorwomen will develop cancer of the cervix if the present bent assay (ELISA) for levels of serum IgG to trend continues . Cervical three human papillomavirus (HPV) peptides screening programmes are inadequate, and a good HPV (HPV-16 E2 [E2-16], HPV-18 E2 (E2-18], HPV-16 vaccine would have a major impact on the incidence of L1 [L1-16]), as well as HPV-16 virus-like particles cervical cancer in this population. Human papillomavi-(VLP-16) and bovine papillomavirus type 1 virusrus type 16 (HPV-16) has been demonstrated to be assolike particles (BPV-VLP). In the adult group anticiated with 46% of cervical cancers and 21% of CIN bodies to E2-16 and VLP-16 were significantly grade 3s (CIN III) in Cape Town [Williamson et al. 1989; associated with CIN when compared to the blood . In these studies the incidence donor controls (P ϭ .039 and P ϭ .002, respecof HPV 18 was low and was detected in only 1.5% of tively). In women with CIN there was an increase cervical cancers and 1% of CIN III biopsies. However, in seropositivity to E2-16 and a decrease in seroin a study of Cape Town women with normal cervical positivity to VLP-16 with age. Antibodies to HPVcytology HPV-18 was the predominant HPV type de-16 E2 could therefore be an important marker of tected . No studies have been CIN in women over 40 years of age, whereas carried out on the prevalence of antibodies to HPV antiantibodies to VLP-16 could be a marker for CIN gens in South Africa. in younger women. There was no correlation
In recent years serological assays for HPV have been with CIN and antibodies to E2-18, L1-16, and BPVdeveloped based on fusion proteins, peptides virions, VLP. In the children's sera antibodies were deand virus-like particles (VLPs) [Bonnez et al., 1991; tected to E2-16 (44.5%), E2-18 (18.7%), L1-16 Cason et al., 1992; Dillner, 1994; Galloway, 1994]. Most (20%), VLP-16 (4.5%), and BPV-VLP (5.1%). Bestudies have attempted to link the seropositivity to spetween the ages of 3 and 12 years the prevalence cific HPV antigens with the disease status of the patient of antibodies to E2-16 decreased with age. The [as reviewed by Dillner, 1994; Schiller and Roden, 1995]. presence of antibodies to HPV-16 in young chil-Recent studies have demonstrated that antibodies to dren indicated infection with either HPV-16 or a
VLPs can be used as markers of infection [Rose et al., related virus. HPV DNA isolation from children 1993