Age-dependent loss of corticosterone modulation of central serotonin 5-HT1A receptor binding sites

✍ Scribed by Lynn W. Maines; B. Jane Keck; Ashish Dugar; Joan M. Lakoski

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 422 KB
• Volume: 53
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/(sici)1097-4547(19980701)53:1<86::aid-jnr9>3.0.co;2-f

✦ Synopsis

A loss of endocrine and neurotransmitter system interactions, including corticosterone regulation of 5-HT 1A receptors, may underlie the age-related deficits in the hypothalamic-pituitary-adrenal (HPA) axis including adapting to stress. In this study, female Fischer 344 rats, (ages 3, 13, and 18 months), were bilaterally adrenalectomized and supplemented for 3 weeks with placebo or corticosterone (200 mg or 600 mg) containing 21 day sustained-release pellets implanted subcutaneously (LC, MC, or HC, respectively). Scatchard analysis using the 5-HT 1A receptor agonist [ 3 H]8-hydroxy-2-(di-N-propylamino) tetralin (8-OH-DPAT) demonstrated a significant decrease in hippocampal receptor density in 3 month and 13 month MC groups (-35.2 and -32.1%, respectively) as compared to age-matched LC groups; a significant decline in 5-HT 1A receptor density in 3 month and 13 month HC groups was found compared to agematched MC groups (؊16.7 and ؊22.0%, respectively). However, these hormone treatments (LC or HC) failed to alter hippocampal 5-HT 1A binding site density in the 18 month groups. Cortical 5-HT 1A receptor densities were altered in a similar agedependent manner. In contrast, the density of hypothalamic 5-HT 1A receptors in the 18 month LC group was significantly increased above that in the 3 month LC group. An additional indicator of the hippocampal response to corticosterone, the distribution of glial fibrillary acidic protein (GFAP), revealed an agerelated decline in responsiveness to hormone treatment in the oldest group. The present study has identified an age-associated deficit in the regulation of hippocampal 5-HT 1A receptors by corticosterone which may underlie the diminished capacity of the aging HPA axis to cope with stress.