Agarose isoelectric focusing for the detection of many isoforms and high molecules in muscle protein analysis

✍ Scribed by Tamio Hirabayashi

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 210 KB
• Volume: 21
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/(sici)1522-2683(20000101)21:2<446::aid-elps446>3.0.co;2-9

✦ Synopsis

Agarose isoelectric focusing for the detection of many isoforms and high molecules in muscle protein analysis Two-dimensional gel electrophoresis with agarose in the first dimension was established. The technique was reported previously and was now much improved without changing the basic procedure, presenting ªa protein mapº of chicken skeletal muscle, including almost all components such as myosin heavy chains (200 kDa) and dystrofin (400 kDa). Application of this technique for the analysis of all components was also successful with the liver and lens. Since large molecules can get into the agarose in the first dimension, the study on protein interaction was also reported with troponin components as a model system.