Aflatoxin B1-induced micronuclei and cell cycle alterations in lung and bone marrow cells and their modulation by Piper argyrophyllum extract
✍ Scribed by Hanumantharao G. Raj; Kshitij Gupta; Viswajeet Rohil; Mridula Bose; Gopa Biswas; Sanjay K. Singh; Subhash C. Jain; Virinder S. Parmar; Carl E. Olsen; Jesper Wengel
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 264 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0270-3211
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✦ Synopsis
Aflatoxin B 1 (AFB 1 ) is a clastogen that causes cellular damage by covalent modification of nucleic acids. In this investigation, male rats were injected i.p. with AFB 1 (8 mg/kg b.w.) in DMSO and the same dose of AFB 1 was also administered intratracheally (i.t.) to the animals separately. The animals were killed after 26 h of the carcinogen treatment, femur bone was removed, and bone marrow cells were isolated and stained with Mayer's hematoxylin and eosin. Micronuclei (Mn) were scored by using light microscopy. Bronchoalveolar lavage (BAL) was prepared from rats administered AFB 1 i.t. A part of BAL was fixed with 70% ethanol, stained with the fluorochrome DAPI, and analysed for cell cycle variations; the other part of the lavage was used for making slides to record Mn with a fluorescent microscope. A significantly greater proportion of lung cells were found to enter cell cycle with extended S-phase due to AFB 1 treatment. Mn were induced in polychromatic erythrocytes (PCE) as compared to normochromatic erythrocytes (NCE) in the bone marrow of AFB 1 -treated rats, where there was nearly a three-fold increase in the number of Mn of bone marrow cells. The administration of AFB 1 resulted in a two-fold rise in the Mn in the lung cells. The effect of BSO, DEM, and PB, the modulators of AFB 1 metabolism, was studied on AFB 1 -induced Mn formation. A significant increase in the Mn score in PCEs of BSO-and DEM-treated rats was noted, while a slight reduction in the Mn score was noted in the case of PB-treated rats. The administration