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Affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag

โœ Scribed by Abdelhamid, Mohamed A. A.; Motomura, Kei; Ikeda, Takeshi; Ishida, Takenori; Hirota, Ryuichi; Kuroda, Akio

Book ID
121822399
Publisher
Springer
Year
2014
Tongue
English
Weight
597 KB
Volume
98
Category
Article
ISSN
1432-0614

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## Abstract The cholineโ€binding domain (ChoBD) of the carboxyโ€terminal region of the __Streptococcus pneumoniae__ amidase LYTA (Cโ€LYTA) presents a strong affinity for tertiary amines. We report a method for singleโ€step purification of proteins expressed in the methylotrophic yeast __Pichia pastoris