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Affinity purification of Hydra glutathione binding proteins

โœ Scribed by Susan L. Bellis; David C. Laux; Dennis E. Rhoads

Book ID
115929591
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
574 KB
Volume
354
Category
Article
ISSN
0014-5793

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Prokaryotic expression of polypeptides as fusion proteins with glutathione-S-transferase has recently been reported as a one-step means of purifying recombinant protein. The usefulness of the glutathione-S-transferase/glutathione- agarose system, however, is significantly limited by the frequent syn