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Affinity purification of gamma-secretase associated proteins in synaptic membranes and synaptic vesicles

✍ Scribed by Frykman, Susanne; Kihara, Takahiro; Yamamoto, Natsuko Goto; Teranishi, Yasuhiro; Winblad, Bengt; Tjernberg, Lars O.


Book ID
122992646
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
46 KB
Volume
6
Category
Article
ISSN
1552-5260

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## Abstract Synaptic vesicles were isolated from adult bovine cortical gray matter by differential centrifugation and membrane filtration of a hypoosmotically lysed crude mitochondrial fraction. Vesicle preparations were analyzed for purity by electron microscopy and enzyme assays. Polyacrylamide g