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Affinity labelling of ribosomal peptidyl transferase by a puromycin analogue

✍ Scribed by R.J. Harris; P. Greenwell; R.H. Symons


Book ID
119405310
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
384 KB
Volume
55
Category
Article
ISSN
0006-291X

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Affinity labelling of yeast ribosomal pe
✍ PΓ©rez-GosΓ‘lbez, Miguel ;VΓ‘zquez, David ;Ballesta, Juan P. G. πŸ“‚ Article πŸ“… 1978 πŸ› Springer 🌐 English βš– 546 KB

Using p-nitrophenylcarbamyl-phenylalanyl-tRNA (PNPC-Phe-tRNA) and N-Iodoacetylphenylalanyl-tRNA as affinity labels we have attempted to identify the components of the aminoacyl-tRNA binding sites located in the vicinity of the peptidyl transferase centre of the yeast ribosome. Both Phe-tRNA derivati