Cysteinyl peptides labeled by dibromobut
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Sara H. Vollmer; Roberta F. Colman
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Article
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1992
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Cold Spring Harbor Laboratory Press
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English
⚖ 967 KB
## Abstract The bifunctional reagent 1,4‐dibromobutanedione (DBBD) reacts covalently with pyruvate kinase from rabbit muscle to cause inactivation of the enzyme at a rate that is linearly dependent on the reagent concentration, giving a second order rate constant of 444 min^−1^ M^−1^. The individua