Affinity binding of the cartilage proteo
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James E. Christner; Martin L. Brown; Dominic D. Dziewiatkowski
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Article
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1978
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Elsevier Science
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English
β 679 KB
The protein-keratan sulfate core of bovine nasal cartilage proteoglycan was purified by affinity chromatography on a column of immobilized hyaluronic acid. The hyaluronic acid was immobilized by reaction with a hydrazido-alkyl derivative of Sepharose in the presence of borohydride. Proteoglycan was