Affinity chromatography on concanavalin a-sepharose of antigenic fractions of human seminal plasma

✍ Scribed by María N. Mazzini; Alberto S. Cerezo; Josefina M.S. de Cerezo

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 569 KB
• Volume: 173
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)92305-7

✦ Synopsis

Elution diagrams obtained on affinity chromatography show that the antigenic fractions of human seminal plasma studied, namely (a) the non-diakzable components of human seminal plasma and (b) its trichloroacetic acid-soluble fraction, (c) the trichloroacetic acid-soluble fraction of whole human seminal plasma and (d) the pronase digested human seminal plasma, are complex mixtures of glycoprqteins with minor amounts of polysaccharides. Some of these glycoproteins contain significant percentages of carbohydrates while others contain only trace amounts. Most of the glycoproteins carry non-reducin, = end-chain groups comprising a-D-glucopyranosyl, a-n-mannopyranosyl or sterically related residues.

& 'isma. The substances were then examined by chemical and physicochemical methods l To whom correspondence should be addressed.