Affinity chromatography of rat plasma albumin on bromosulfophthalein-glutathione conjugate covalently linked to Sepharose 4-B
✍ Scribed by Alan G. Clark; Wong Sie Ting
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 292 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
In a recent publication (I), we described a matrix for the affinity chromatography of glutathione S-transferase. The matrix was bromosulfophthalein-glutathione conjugate covalently linked to Sepharose 4-B. It was observed in this publication that this matrix could be used for the preparation of rat plasma albumin and we now wish to describe this application in greater detail and to compare the properties of this matrix with those of matrixes described by other workers.
MATERIALS
Sulfobromophthalein
and L-glutathione were purchased from Koch-Light Laboratories Ltd. and CNBr-activated Sepharose 4-B from Pharmacia.
Blood was taken from ether-anaesthetized rats of both sexes into a heparinized syringe from the posterior vena cava. It was centrifuged for 20 min at lOOO,r: at room temperature. The supernatant was decanted and stored at -20°C until required.