The Use of Ni–Nitrilotriacetic Acid Agar
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Andrey A. Komissarov; Marie T. Marchbank; Susan L. Deutscher
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Article
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1997
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Elsevier Science
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English
⚖ 177 KB
The complex formed between 32P-labeled (dT)15 and a hexahistidine (6-His)-tagged anti-single-stranded DNA (ssDNA) Fab, DNA-1, was trapped by addition of nickel-chelating nitrilotriacetic acid (Ni-NTA) agarose that led to efficient separation of bound ligand from free. High stability of the immobiliz