AF6 gene on chromosome band 6q27 maps distal to the minimal region of deletion in epithelial ovarian cancer
✍ Scribed by Vaskar Saha; Debra M. Lillington; Andrew N. Shelling; Tracy Chaplin; Marie-Laure Yaspo; Trivadi S. Ganesan; Professor Bryan D. Young
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 266 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1045-2257
No coin nor oath required. For personal study only.
✦ Synopsis
Chromosome 6 has been shown to contain at band q27 a minimal region of deletion associated with epithelial ovarian cancers and AF6, a gene disrupted in acute myeloid leukemia with t(6;l l)(q27;q23). Using rapid amplification of cDNA ends by polymerase chain reaction, the breakpoint in AF6 was confirmed and a cDNA clone identified. This clone was used as a probe to screen a chromosome 6 cosmid library, and a single cosmid C-I09F0645 was isolated. By fluorescence in situ hybridization, C-109FO465 was found to map distal to the critically deleted region associated with ovarian malignancies. AF6 is therefore distinct from and lies telomeric to this region. Genes Chrornosorn Cancer 14: 220-222 (1995).