Aerosol-generated sol-gel-derived thin films as biosensing platforms
✍ Scribed by Jeffrey D. Jordan; Richard A. Dunbar; Frank V. Bright
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 842 KB
- Volume
- 332
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
We have incorporated an antibody into a sol-gel-derived thin film composite and demonstrated that it retains affinity for its hapten. An inexpensive apparatus to produce and deposit, aerosol-generated sol-gel-derived thin films was also developed. The antibody antifluorescein (AF) was entrapped between two sol-gel thin films (0.62f0.05 pm) in a sandwich architecture. The fluorescent hapten 5-(-and 6-)-carboxy-4',5'-dimethylfluorescein (MeaF) was used to determine the accessibility and viability of the entrapped AF. Results demonstrate that a population of the entrapped antibodies recognizes and binds MeaF for up to 13 weeks when stored in pH 8 phosphate buffer at 4°C. An intriguing result is that the response times of these AF-doped thin films actually improve as a function of storage time. We explain this observation in terms of decreased viability of AP molecules sequestered between the upper and lower films of the sandwich. Partial regeneration (40-50%) of the biosensor was obtained using a mild chaotropic reagent (NaCI) and the AF-doped films could be reset/regenerated up to six times.