Aerobic inhibition of nitrate assimilation in Escherichia coli

✍ Scribed by Miya Kobayashi; Prof. Dr. M. Ishimoto

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 566 KB
• Volume: 13
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19730130505

✦ Synopsis

Abstract

Growth of Escherichia coli, strain K‐10, in synthetic medium with nitrate as a sole source of nitrogen was not found under aerobic conditions, but found in the medium under anaerobic conditions as well as in synthetic medium with ammonium or nitrite under both aerobic and anaerobic conditions. The presence of nitrate reductase activity in cells indicates that the growth inhibition is due to the inhibition of the enzyme by oxygen and not due to its absence. Conditions of preculture, inoculation size, pH, and composition of the nitratesynthetic medium did not affect the inhibition. All the 37 strains of E. coli tested were grown in the nitrate medium only anaerobically, while 18 strains of Klebsiella‐Aerobacter among 22 strains tested were grown in the nitrate medium both aerobically and anaerobically.