Adventitious bud regeneration from leaf expiants of the shrubby ornamental honeysuckle,Lonicera nitidaWils. cv. ‘Maigrün’: effects of thidiazuron and 2,3,5-triiodobenzoic acid

✍ Scribed by Jocelyne Cambecèdes; Michel Duron; Luc Decourtye

• Book ID: 104675564
• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 628 KB
• Volume: 10
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00233817

✦ Synopsis

Different combinations of auxins and cytokinins were employed to assess the regeneration capacity from in vitro leaf explants of Lonicera nitida Wils. cv 'Maïgrün'. A high frequency of rhizogenesis was noticed, with 2.3 μM thidiazuron plus 2.9 μM indole-3-acetic acid as the only hormonal combination to support caulogenic responses. Increasing thidiazuron concentration and/or suppressing auxin did not improve caulogenesis. Combining thidiazuron with 2,3,5-triiodobenzoic acid produced a dramatic increase in the percentage of caulogenic explants. A maximum of 74% of adventitious bud forming explants was obtained with 2.3 μM thidiazuron plus 20 μM 2,3,5-triiodobenzoic acid. Buds were often in a rosette form and were vitreous, so that shoot elongation was difficult to obtain. The effect of the duration of the 2,3,5-triiodobenzoic acid treatment on shoot elongation was investigated.