✦ LIBER ✦
Advantages of continuous over batch reactors for the kinetic analysis of enzymes inhibited by an unknown substrate impurity
✍ Scribed by Alberto Gallifuoco; Francesco Alfani; Maria Cantarella
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 144 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
✦ Synopsis
A new experimental technique, employing a continuous stirred-tank reactor, for studying enzyme kinetics in the presence of inhibitor-contaminated substrate is described. The proposed method is simulated mathematically for competitive, uncompetitive, and mixed-type noncompetitive inhibition. The step-by-step experimental procedure is described, as is the necessary data analysis for determining the kinetic parameters. Differences in system response for enzyme inhibition by excess substrate and by an impurity are illustrated, and a stability analysis of the system is performed.