Advances in Tracer Methodology: Volume 1

✍ Scribed by F. Marott Sinex (auth.), Seymour Rothchild (eds.)

• Publisher: Springer US
• Year: 1995
• Tongue: English
• Leaves: 341
• Edition: 1
• Category: Library

✦ Synopsis

The five Symposia on Advances in Tracer Methodology were held annually from 1957 to 1961. The symposia were directed to scientists who are active in utilizing tracer techniques to help solve their scientific problems. The format, an informal one-day meeting consisting of about ten papers and closing with a cocktail hour, fostered an active exchange of information among speakers and audience. Although the first two symposia were restricted to the use of tritium as a tracer isotope, the larger purpose of the meetings was to disseminate information relating to the entire isotopic tracer field. The sponsoring organizations, all actively engaged in selling products in the nuclear field, attempted to provide a noncom­ mercialized forum which would facilitate this exchange of in­ formation. The collection of papers presented herein represents most of the talks presented at the first symposia plus several appropriate papers which have appeared either in Atomlight, the bulletin of the New England Nuclear Corp., or which have been submitted directly for inclusion in this collection. Although each of the authors was given the opportunity to revise his paper, it is likely that some of the techniques or instrumentation described may already have been outmoded by recent improvements.

✦ Table of Contents

Front Matter....Pages i-xi
Opening Remarks by Chairman....Pages 1-3
The Gas Exposure Technique for Tritium Labeling....Pages 4-11
Experiences with Tritiated Compounds Prepared by Exposure to Tritium Gas....Pages 12-17
Reaction of Unsaturated Organic Compounds with Tritium Gas....Pages 18-27
Tritium Gas Exposure Labeling....Pages 28-31
Some Experiences with Labeling Sugars by Tritium Gas Exposure....Pages 32-35
Some Experiences with Labeling Nucleotides....Pages 36-41
Correlation of Efficiency of Labeling with Chemical Constitution....Pages 42-45
The Radiation-Induced Addition of Tritium to Unsaturated Systems....Pages 46-49
Tritium Labeling by Other Methods....Pages 50-51
Tritium Recoil Labeling of Linseed Oil....Pages 52-55
Preparation of Tritium-Labeled Paromomycin (Humatin) by Fermentation in a Medium Containing Tritiated Water....Pages 56-59
Some Aspects of Stereoselectivity in the Introduction of Tritium into Steroids....Pages 60-63
Radiation Self-Decomposition of Labeled Compounds....Pages 64-68
Liquid Scintillation Counting of Tritium....Pages 69-75
Recent Developments in Simultaneous C 14 and Tritium Counting....Pages 76-85
Determination of Liquid Scintillation Counting Efficiency by Pulse-Height Shift....Pages 86-92
A New Technique for Counting Aqueous Solutions in the Liquid Scintillation Spectrometer....Pages 93-105
Liquid Scintillation Counting of Tritium in Suspended Materials....Pages 106-112
Counting C 14 O 2 with a Liquid Scintillation Counter....Pages 113-114
Liquid Scintillation Counting of C 14 O 2 in Aqueous Carbonate Solutions....Pages 115-118
Determination of Radioactive Sulfur in Biological Materials....Pages 119-120
Scintillation Counting of C 14 -Labeled Paper Chromatograms....Pages 121-126
Simultaneous Measurement of C 14 and H 3 during Gas-Liquid Chromatography....Pages 127-146
Monitoring Gas Chromatography for H 3 - and C 14 -Labeled Compounds by Liquid Scintillation Counting....Pages 147-152
Application of Whole-Body Liquid Scintillation Counters to Pharmacological Studies....Pages 153-159
A Comparison of Ionization-Chamber and Liquid-Scintillation Methods for Measurement of Beta Emitters....Pages 160-166
Tritium Measurement Using Ionization Chambers....Pages 167-177
Gas Counting of Tritium....Pages 178-182
Vapor Phase Proportional Counting....Pages 183-184
Determination of Tritium and C 14 in Biological Samples by Rapid Combustion Techniques....Pages 185-191
Parr Bomb Combustion of Tissues for C 14 and Tritium Analysis....Pages 192-194
Recent Advances in Instrumentation....Pages 195-200
Tritium Health Physics Considerations....Pages 201-202
Design and Analysis of Tracer Experiments....Pages 203-204
Design and Analysis of Tracer Experiments—Biochemical Aspects....Pages 205-209
Experimental Design of Turnover Measurement Studies in the Unsteady State: Biosynthesis of Macromolecules....Pages 210-216
Measuring Turnover Rates in the Nonsteady State....Pages 217-221
Tritium in Biochemical Studies....Pages 222-226
Special Features of Tritium as a Tracer in Industrial Research....Pages 227-233
Some Uses of Tritium-Labeled Compounds in Pharmaceutical Research....Pages 234-246
Metabolism of DL-Epinephrine-7-H 3 D-Bitartrate....Pages 247-256
Application of Tritium in the Determination of Gibberellins....Pages 257-262
New Developments in the Use of Radioisotopes in Reservoir Engineering....Pages 263-264
Use of Radioisotopes in Steroid Methodology....Pages 265-273
Metabolism Studies by Radiorespirometry....Pages 274-290
Autoradiography with Tritium....Pages 291-294
Some Uses of Tritium in Autoradiography....Pages 295-301
The Efficiency of Autoradiographic Stripping Film Applied to Tissue Sections Containing Tritiated Thymidine....Pages 302-308
Autoradiography of Whole Animals as an Experimental Tool in Pharmacological Research....Pages 309-313
Clinical Use of the Arm Counter in Blood Clearance Studies....Pages 314-325
Back Matter....Pages 327-332

✦ Subjects

Physical Chemistry