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Advances in lentiviral vector design for gene-modification of hematopoietic stem cells

✍ Scribed by Aaron C Logan; Carolyn Lutzko; Donald B Kohn


Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
84 KB
Volume
13
Category
Article
ISSN
0958-1669

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✦ Synopsis


Lentiviral vectors are more efficient at transducing quiescent hematopoietic stem cells than murine retroviral vectors. This characteristic is due to multiple karyophilic components of the lentiviral vector pre-integration complex. Lentiviral vectors are also able to carry more complex payloads than murine retroviral vectors, making it possible to deliver expression cassettes that direct either constitutive or targeted expression in various hematopoietic stem cell progeny.