✦ LIBER ✦

Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins

✍ Scribed by Aaron L Kurtzman; Sridhar Govindarajan; Katherine Vahle; Jennifer T Jones; Volker Heinrichs; Phillip A Patten

Book ID: 104361579
Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 102 KB
Volume: 12
Category: Article
ISSN: 0958-1669
DOI: 10.1016/s0958-1669(00)00228-7

No coin nor oath required. For personal study only.

✦ Synopsis

Recent developments in directed evolution technologies combined with innovations in robotics and screening methods have revolutionized protein engineering. These methods are being applied broadly to many fields of biotechnology, including chemical engineering, agriculture and human therapeutics. More specifically, DNA shuffling and other methods of genetic recombination and mutation have resulted in the improvement of proteins of therapeutic interest. Optimizing genetic diversity and fitness through iterative directed evolution will accelerate improvements in engineered protein therapeutics.

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