Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins
✍ Scribed by Friederike Junge; Stefan Haberstock; Christian Roos; Susanne Stefer; Davide Proverbio; Volker Dötsch; Frank Bernhard
- Book ID
- 108218483
- Publisher
- Elsevier
- Year
- 2011
- Tongue
- English
- Weight
- 530 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1871-6784
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For the application of multidimensional NMR spectroscopy to larger proteins, it would be useful to perform selective labeling of one of the 20 amino acids. For some amino acids, however, amino acid metabolism drastically reduces the efficiency and selectivity of labeling in in vivo expression system